What are the spectrometry methods for analyzing CAS 56-75-7?

As a supplier of CAS 56 - 75 - 7, I've had the privilege of engaging with a diverse clientele in the chemical and pharmaceutical industries. CAS 56 - 75 - 7, which is Chloramphenicol, is a well - known antibiotic with a long - standing history in the medical field. In this blog, I'll delve into the spectrometry methods used for analyzing this important compound.

1. Overview of CAS 56 - 75 - 7 (Chloramphenicol)

Chloramphenicol is a broad - spectrum antibiotic that was first isolated from the bacterium Streptomyces venezuelae in 1947. It has been used to treat a variety of bacterial infections, including typhoid fever, meningitis, and certain eye infections. However, due to its potential side effects, such as aplastic anemia, its use has become more restricted in recent years. Despite this, accurate analysis of Chloramphenicol remains crucial for quality control in production, research, and regulatory compliance.

2. Mass Spectrometry (MS)

Mass spectrometry is a powerful analytical technique that can provide information about the molecular weight and structure of a compound. In the analysis of Chloramphenicol, MS can be used to confirm its identity and purity.

Principle

The basic principle of mass spectrometry involves ionizing the sample molecules and then separating the ions based on their mass - to - charge ratio (m/z). The ions are detected, and a mass spectrum is generated, which shows the relative abundance of each ion as a function of m/z.

Application in Chloramphenicol Analysis

For Chloramphenicol, electron ionization (EI) or electrospray ionization (ESI) can be used. EI is a hard ionization technique that can produce a characteristic fragmentation pattern. The molecular ion peak of Chloramphenicol at m/z 323 can be identified, along with other fragment ions that provide clues about its structure. ESI, on the other hand, is a soft ionization technique that is more suitable for analyzing thermally labile or polar compounds like Chloramphenicol. It can produce mainly protonated or deprotonated molecular ions, which are useful for accurate mass determination.

When combined with chromatography, such as liquid chromatography - mass spectrometry (LC - MS) or gas chromatography - mass spectrometry (GC - MS), the separation of Chloramphenicol from other impurities can be achieved before mass analysis. This allows for more accurate quantification and identification of the compound in complex matrices. For example, in food samples, LC - MS can be used to detect trace amounts of Chloramphenicol residues, as it has high sensitivity and selectivity.

3. Nuclear Magnetic Resonance (NMR) Spectroscopy

NMR spectroscopy is another important tool for analyzing the structure of organic compounds, including Chloramphenicol.

Principle

NMR spectroscopy is based on the interaction of atomic nuclei with an external magnetic field. When a sample is placed in a magnetic field, certain nuclei (such as ¹H and ¹³C) can absorb radiofrequency radiation and undergo a transition between different energy levels. The resulting NMR spectrum provides information about the chemical environment of the nuclei, which can be used to determine the molecular structure.

Application in Chloramphenicol Analysis

In the analysis of Chloramphenicol, ¹H NMR and ¹³C NMR spectra can be obtained. The ¹H NMR spectrum can show the number and types of hydrogen atoms in the molecule, as well as their relative positions. For example, the aromatic protons in Chloramphenicol will appear in a characteristic chemical shift range, and the coupling patterns between the protons can provide information about the connectivity of the atoms. The ¹³C NMR spectrum, on the other hand, can provide information about the carbon skeleton of the molecule.

Two - dimensional NMR techniques, such as correlation spectroscopy (COSY) and heteronuclear multiple - bond correlation (HMBC), can be used to further elucidate the structure of Chloramphenicol. These techniques can help to establish the connectivity between different atoms in the molecule and confirm the proposed structure.

4. Infrared (IR) Spectroscopy

IR spectroscopy is a simple and rapid method for identifying functional groups in a compound.

Principle

IR spectroscopy measures the absorption of infrared radiation by a sample. Different functional groups in a molecule absorb infrared radiation at characteristic frequencies, which are related to the vibrational modes of the bonds. The resulting IR spectrum shows a series of peaks at specific wavenumbers, which can be used to identify the functional groups present in the compound.

Application in Chloramphenicol Analysis

In the case of Chloramphenicol, the IR spectrum can be used to identify the presence of functional groups such as amide, nitro, and hydroxyl groups. The amide carbonyl group (C = O) will show a strong absorption peak around 1650 - 1680 cm⁻¹, while the nitro group (NO₂) will have absorption peaks around 1500 - 1550 cm⁻¹ and 1300 - 1380 cm⁻¹. The hydroxyl group (OH) will show a broad absorption peak around 3200 - 3600 cm⁻¹. By comparing the IR spectrum of a sample with the reference spectrum of Chloramphenicol, the identity of the compound can be confirmed.

5. Ultraviolet - Visible (UV - Vis) Spectroscopy

UV - Vis spectroscopy is mainly used for the quantification of Chloramphenicol.

Principle

UV - Vis spectroscopy measures the absorption of ultraviolet and visible light by a sample. The absorption of light by a molecule is related to the electronic transitions between different energy levels. The absorbance of a sample at a specific wavelength is proportional to the concentration of the absorbing species, according to the Beer - Lambert law.

Application in Chloramphenicol Analysis

Chloramphenicol has a characteristic absorption peak in the UV region at around 278 nm. By measuring the absorbance of a sample at this wavelength, the concentration of Chloramphenicol can be determined. This method is relatively simple and can be used for routine quality control in the production of Chloramphenicol. However, it is important to note that other compounds in the sample matrix may also absorb at the same wavelength, which can lead to interference. Therefore, proper sample preparation and calibration are necessary.

6. Related Compounds as References

When analyzing Chloramphenicol, it can be helpful to compare its spectra with those of related compounds. For example, Azithromycin CAS# 83905 - 01 - 5 is another well - known antibiotic. Although its structure is different from Chloramphenicol, comparing their spectra can provide insights into the spectral characteristics of antibiotics in general.

BENZOIC ACID, 2 - [[(1,1 - DIMETHYLETHOXY)CARBONYL]AMINO] - 3 - NITRO - METHYL ESTER CAS#57113 - 90 - 3 contains some similar functional groups to Chloramphenicol, such as the nitro group. Analyzing its spectra can help in understanding the spectral behavior of these functional groups in different chemical environments.

Beta - Nicotinamide Mononucleotide CAS#1094 - 61 - 7 is a bioactive compound. Although it is not directly related to Chloramphenicol in terms of function, comparing its spectrometry data can expand our knowledge of different types of organic compounds.

7. Conclusion and Call to Action

In conclusion, the spectrometry methods described above play a vital role in the analysis of CAS 56 - 75 - 7 (Chloramphenicol). Mass spectrometry provides information about the molecular weight and structure, NMR spectroscopy helps to elucidate the detailed molecular structure, IR spectroscopy identifies functional groups, and UV - Vis spectroscopy is used for quantification.

As a supplier of CAS 56 - 75 - 7, we are committed to providing high - quality Chloramphenicol products. Our products are analyzed using these advanced spectrometry methods to ensure their purity and quality. If you are interested in purchasing Chloramphenicol or have any questions about its analysis, please feel free to reach out to us for procurement negotiations. We look forward to collaborating with you to meet your specific needs.

References

1. Smith, J. K. (2015). Analytical Chemistry of Antibiotics. CRC Press.
2. Gross, M. L. (2017). Mass Spectrometry: A Textbook. Springer.
3. Silverstein, R. M., Webster, F. X., & Kiemle, D. J. (2014). Spectrometric Identification of Organic Compounds. Wiley.